RM21™ Microscope Platform
- Microscope automatic focus drift correction
- Interchangeable, quick mount adapters
- 100 μm range of motion
- Compatible with all microscopes
- Closed loop control
- Maintain constant microscope focus
- High speed confocal imaging
- Ultra-fine focus adjustments
- Drift correction
C-Focus™ systems provide an automatic means to eliminate
microscope focus drift over long time periods.
Unlike autofocus systems which require use of advanced
microscopes with internal focus correction or external
devices which track video signals or reflected laser beams,
the C-Focus™ simply corrects for microscope focus drift by
using a high resolution (5nm) sensor system to measure
the lens/sample spacing and make the necessary adjustments.
The C-Focus™ system has no effect on standard
manual focusing and can be retrofit to any microscope.
After focus is established, simply pushing the “focus lock”
button starts the C-Focus™ operation. Objective lens motions
are accomplished with a lens nanopositioner (the Nano-F100S) which is easily connected to the objective
lens and microscope with standard threaded adapters.
In addition to the unique C-Focus™ operation, lens nanopositioner
motion can also be directly commanded by
digital (16-bit USB) or analog signals and can be used
for high speed, high resolution confocal imaging and
other imaging tasks with demanding focus requirements.
The C-Focus™ lens positioning system contains the same
proprietary, low noise PicoQ® position sensors used in
the Nano-F100S and is capable of sub-nanometer positioning
resolution. The 16-bit USB digital computer
interface is included with all C-Focus™ controllers.
Set of Cy3 single molecule imaging
Sample: MutS protein imaged with Cy3 in presence of oxygen scavenging system
Technique: Prism TIRF
Microscope: Olympus IX71
Objective: 60x 1.2 NA Water Immersion, 1.6x magnification
Camera: Hamamatsu EMCCD
Biomolecular & Cellular Dynamics Lab
Dept of Physics/IBIO, POSTECH South Korea
Focus Drift vs. Constant Focus
|Range of motion (Nano-F100)
||500 Hz ±20%
|Recommended max. load*
||Al and Brass
|* Larger load requirements should be discussed with our engineering staff.