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Single Molecule Microscopy
>>MicroMirror TIRF System

Additional Information
Catalog Pages
Nature Protocols Paper

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TIRF Module
TIRF Lock
Nano-Cyte®
Accessories
Nano-Drive®

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Features
  • Integrated piezo nanopositioning system
  • Integrated imaging & motion control platform
  • Spatially separated excitation & emission beams
  • Adjustable micromirrors
  • Open access to optical pathways
  • Highly stable, yet flexible, platform
  • Integrated slide holder
  • TIRF Lock feedback control
Advantages
  • Superior signal-to-noise ratio
  • Eliminates dichroic mirrors
  • Optical pathway accessibility
  • Facilitates multiple laser lines
  • Proven design
  • Enabling technology for studying complex biomolecular interactions
  • Commercial solution



Product Description

The Mad City Labs' MicroMirror TIRF microscope is part of our RM21™ microscope family and draw on our expertise with high precision, high stability piezo nanopositioning systems and microscopy solutions. The microscopy technique of colocalization single molecule spectroscopy (CoSMoS) is the only proven method to study the ordered assembly and function of multicomponent biomolecular machines. The MicroMirror TIRF microscope is the only commercially available CoSMoS microscope.

The MicroMirror TIRF microscope uses through-theobjective excitation, but replaces the dichroic used in conventional TIRF systems with two broadband micro-mirrors positioned at the back aperture of the objective lens. Spatially separating the excitation and emission beams and eliminating the dichroic mirror leads to superior signal-to-noise ratios and simplifies the introduction of multiple spectral lines to your experiment. Typical biomolecular systems studied using micro-mirror TIRF incorporate 3 or more laser lines which can be difficult to accomodate in conventional dichroic TIRF instruments. Each micro-mirror is mounted to a multi-axis precision mount allowing the user to make fine adjustments to the optical pathway. As with all RM21™ microscopes it is designed to maximize optical pathway accessibility and flexibility, while also simplifying the optical alignment.

A compelling advantage of our MicroMirror TIRF microscope the open access to the entry and exit optical pathways, integrated XYZ closed loop piezo nanopositioning system. The XYZ closed loop nanopositioner uses proprietary PicoQ® sensors for sub-nanometer precision and high stability. A z-axis micropositioner with integrated sample holder is mounted above the piezo nanopositioning system.

Completing our MicroMirror TIRF microscope is the optional TIRF Lock™ feedback control consisting of a QPD sensor, TIRF Lock controller and software. The TIRF Lock module maintains the TIR signal through software feedback to the piezo nanopositioner. The MicroMirror TIRF microscope is a proven design and offers a simple yet flexible instrument platform for single molecule research.


Compatible Software Packages:
      





Technical Specifications - Imaging & Motion Control

Micropositioning motion XYZ
Ranges of motion (XY)
25 mm
Range of motion (Z) 10 mm
Body Material Anodized Aluminum
Nanpositioning Motion XYZ
Ranges of motion (XYZ) 200 μm
Resolution 0.4 nm
Recommended max. load (horizontal)* 0.5 kg
Body Material Aluminum
Controller† Nano-Drive®
Digital Interface USB 2.0
Analog Input 0 to 10V
Synchronization outputs TTL (4)
Nanopositioner Software† Nano-Route®3D
TIRF Software compatibility GLIMPSE

Technical Specifications - MicroMirror Mounts

Micro-Mirror motion 5 axis
Micro-Mirror diameter 3 mm
Mount material Aluminum


Technical Specifications - TIRF Lock™ Feedback

TIRF-Lock™ Sensor Quadrant Photodiode
Controller TIRF Lock
Digital interface USB 2.0
Software TIRF Lock


* Larger load requirements should be discussed with our engineering staff.
† See Software Compatibility, above


Additional Information

MicroMirror TIRF Catalog Pages
Design and construction of a multiwavelength, micromirror total internal reflectance fluorescence microscope. Larson, J. et al. Nature Protocols 9, 2317–2328 (2014)


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