• Unique MicroMirror TIRF modality ideal for multi-spectral single molecule microscopy
  
• Spatially separated excitation & emission beams giving superior signal-to-noise ratios
  
• High stability microscopy platform with open access to optical pathways - Ideal for future innovation
  
• Integrated high resolution, closed loop nanopositioning
• 3rd Generation Micromirrors with synchronized motorized movement & software control
  
• Options: TIRF-lock^® control, dichroic TIRF mode, multiview imaging
• Includes pre-installation support, on-site installation & training

Product Description

The RM21^® MicroMirror TIRF microscope was developed specifically for multispectral single molecule microscopy. Techniques such as colocalization single molecule spectroscopy (CoSMoS) study the ordered assembly and function of multi-component biomolecular machines and require exceptional signal-to-noise ratios.
This microscope is the only commercially available MicroMirror TIRF microscope.

The MicroMirror TIRF microscope uses through-the-objective excitation, but replaces the dichroic used in conventional TIRF systems with two broadband micromirrors positioned at the back aperture of the (fixed)objective lens. Spatially separating the excitation and emission beams and eliminating the dichroic mirror leads to superior signal-to-noise ratios and simplifies the introduction of multiple spectral lines to your experiment. Typical biomolecular systems studied using micromirror TIRF incorporate three or more laser lines which can be difficult to accommodate in conventional dichroic TIRF microscopes.

Each micromirror is installed on a factory calibrated manipulator designed to place the micromirrors in close proximity to the back aperture of the objective lens. The micromirrors are stepper motor driven and are controlled via software. Setpoints for different TIRF angles can be saved in software for easy return during experiments. Moreover, the final focusing lens just prior to the entrance micromirror moves in synchrony with these positional changes. The micromirrors can be moved independently to facilitate alignment procedures or experimental needs. When micromirror TIRF is not used on the microscope, the mirrors can be manually retracted.

The RM21^® MicroMirror TIRF microscope is part of the RM21^® single molecule microscope family. These microscopes have been designed for users who wish to adapt and innovate techniques on their instruments - in essence a sophisticated "do-it-yourself" microscope.

Our RM21^® microscopes are designed to maximize optical access and adaptability, simplify optical alignment, and optimize stability for single molecule microscopy and nanoscopy applications. The microscope has been designed for use with piezo nanopositioners. The inclusion of our closed loop nanopositioners with proprietary PicoQ^® sensors ensures high precision and high stability of sample motion.

Compatible Software^† Packages:
      

Technical Specifications - Sample Control

Technical Specifications - MicroMirrors

Technical Specifications - General

Additional Information

References

Related Products

• RM21^® Microscopes
• TIRF Module
• TIRF-Lock^®
• Nano-Cyte^®
• Accessories
• Nano-Drive^®