As part of our Single Molecule Imaging portfolio, Mad City Labs offers a MicroMirror TIRF microscopy system. This system enables scientists to study the ordered assembly and function of biological complexes at the single molecule level.

Our MicroMirror TIRF system uses through-the-objective excitation, but replaces the dichroic mirror used in conventional TIRFM systems with two broadband micromirrors positioned at opposite edges of the back aperture of the objective lens. This illumination method enables the spatial separation of the excitation and emission pathways, eliminating the need to spectrally separate them using a dichroic mirror. By eliminating multi-band dichroic mirrors, our MicroMirror TIRF microscopy system yields the superior photon sensitivity and signal-to-noise ratios required for single molecule analyses using three or more fluorophores.

For applications requiring TIR illumination over long periods of time, Mad City Labs has also developed a "TIRF-lock" system. The TIRF-lock software adjusts the Z-axis position of the nanopositioner to maintain the desired TIR illumination angle.